Agarose Gel Electrophoresis ee RNA

Daraasad cusub oo ka timid RNA

Dhawaan, cilmi-baaris ayaa ogaatay in kala duwanaanshaha hidda-socodkakuwaas oo hoos u dhigaya heerarka tafatirka ee RNA laba-jibbaaran waxay la xiriiraan difaaca jirka iyo xaaladaha isdifaaca.

Unugyada RNA waxay mari karaan isbeddel. Tusaale ahaan, nucleotides waa la gelin karaa, la tirtiri karaa, ama waa la beddeli karaa. Mid ka mid ah tafatirrada ugu caansan, oo cilmi-baaris cusub ay muujisay inay door muhiim ah ka ciyaarto bilawga cudurrada bararka, waa isbeddelka adenosine nucleotide oo loo beddelo inosine gudaha RNA laba-geesood ah. Daraasadahaala daabacayon Agoosto 3 ee Dabeecadda waxay muujineysaa in kala duwanaanshaha hidda-socodka ee yareynaya isbeddelkan gaarka ah ay la xiriirto khatarta sii kordheysa ee cudurrada bararka ee difaaca jirka iyo difaaca jirka sida psoriasis, cudurka mindhicirka bararka, iyo nooca 1aad ee sonkorowga. Qorayaashu waxay soo jeedinayaan in borotiinka dareemayaasha ay u badan tahay inuu khalado RNA-yadan aan tafatirin ee molecules shisheeye, taasoo kicinaysa jawaab-celin barar ah.

Biologist molecular ayaa u maleeyay in'runtii waa horumar weyn oo la helaywaxa ay "runtii soo jiidatay" sida ay muhiim ugu tahay tafatirka RNA ee cudurada bararka, marka la barbar dhigo muujinta hiddasidaha ama kala qaybinta.

Runtii waa cilmi baaris weyn. Iyo iibka alaabada elektiroonigga ah, waxaan aad u xiiseynayaa waa maxay RNA, iyo sida saynisyahannadu u falanqeeyaan RNA by electrophoresis? Runtii waa aqoon aad u xirfad badan oo aan la aqoon laakiin wali way xiiso badan tahay inaan sahamiyo.

Waa maxay RNA?

RNA, oo gaaban Ribonucleic acid, aashitada nucleic ee ku jirta dhammaan unugyada noolaha. Si ka duwan DNA-da, RNA waa inta badan hal xarig. Doorkeeda ugu muhiimsan waa inuu u dhaqmo sidii fariin sidday tilmaamo DNA ah oo ku saabsan xakameynta isku dhafka borotiinka, in kasta oo fayrasyada qaarkood RNA halkii DNA ay qaadi lahaayeen macluumaadka hidda-socodka.

Agarose Gel Electrophoresis ee RNA

Dadku way awoodaanqiimee tayada RNA by jel electrophoresis.

Hal qayb oo ka mid ah Beijing Liuyi Biotechnology Co.Ltdsoo-saare xirfad leh oo diiradda saaraya alaabada elektrophoresis ee ka badan 50 sano, waxay bixisaa taangiyo kala duwan oo elektrophoresis ah (qolal / unugyo) oo loogu talagalay gel electrophoresis, sida taangiyada elektiroonigga ah ee jiifka ah (qolal / unugyo) loogu talagalay agarose gel electrophoresis, iyo taangiyada elektrophoresis toosan (qolal / unugyo) borotiinka jel electrophoresis. Dhanka kale, waxay sidoo kale bixisaa noocyo kala duwan oo koronto koronto ah, golaha golaha mugdiga ah ee sanduuqa mugdiga ah iyo transilluminator UV. Waxaad dooran kartaa Beijing Liuyi's electrophoresis taangiyada (qolalka/unugyada) si loo cabbiro miisaanka molecular ee RNA iyo in la kala saaro RNA ee cabbirka kala duwan ee shaybaarkaaga.

Halkan waxaan ku soo bandhigi doonaa hal nooc oo ah taangiga elektrophoresis jiifka ah (qol / unug) model DYCP-31DN ka our 31 taxane alaabta.

DYCP-31DN waa taangiga elektrophoresis toosan (qol/unug) cabbir yar oo lagu cabbiro miisaanka kelli ee RNA ama DNA iyo in la kala saaro.