1. Kala saarid
Gel electrophoresis wuxuu u qaybsan yahay noocyo toosan (oo ay ku jiraan jeladaha tiirarka iyo jeladaha sariiraha) iyo noocyo toosan (gaar ahaan jeelyada sariiraha) (Jaantuska 6-18). Guud ahaan, kala-soocidda toosan ayaa waxyar ka sarreeya dhinaca toosan, laakiin diyaarinta gelitaanka jiifka ah waxay leedahay ugu yaraan afar faa'iidooyin: waxaa jira taageero ka hooseeya jel oo dhan, taas oo u oggolaanaysa isticmaalka agaroose-fiirsashada hoose; waxaa suurtagal ah in la diyaariyo taarikada gel agarose ee sifooyin kala duwan; diyaarinta jel iyo loading muunad ayaa aad ugu habboon; qolka electrophoresis waa sahlan tahay in la dhiso oo kharash-ool ah. Maaddaama electrophoresis jiifka ah lagu sameeyo saxanka agarose jel si buuxda u quusay oo ku saabsan 1mm ka hooseeya dusha sare ee electrophoresis buffer, waxa kale oo loo yaqaan electrophoresis submerged.
Taangiga Electrophoresis DYCP-31DN ee agarose gel electrophoresis
2.Nidaamka Buffer
Kala soocida aashitada nukliyeerka, nidaamyada badankood waxay qaataan habab joogto ah. Qalabka elektrophoresis ee sida caadiga ah loo isticmaalo waxaa ka mid ah TBE (0.08mol/L Tris·HCl, pH 8.5, 0.08mol/L boric acid, 0.0024mol/L EDTA) kaydiyaha iyo THE (0.04mol/L Tris·HCl, pH 7.8, 0.02mol/L sodium acetate, 0.0018mol/L EDTA) kaydiye. Buffers-yadan waxaa guud ahaan loo diyaariyey sidii 10x xalal saami ah waxaana lagu qasi jiray u-fiirsashada loo baahan yahay marka la isticmaalayo. Heerarka socdaalka ee DNA-da toosan iyo wareega ah ee gel agarose waxay ku kala duwan yihiin kaydiyaha la isticmaalay. Badbaadiyaha dhexdiisa, heerka guuritaanka DNA-da toosan wuu ka weyn yahay kan DNA-da wareegtada ah, halka kaydka TBE, lidku yahay.
3.Diyaarinta Agaroose Gel
(1) Diyaarinta Horizontal Agarose Gel
(a) Diyaari fiirsashada loo baahan yahay ee jel agarose adiga oo isticmaalaya 1x electrophoresis buffer.
(b) Kuleyli agaroosku si uu u dhamaystiro milanka ha ahaato qubeyska biyaha karkaraya, kiciya birlabeedka, ama microwave-ka. Qabooji xalka agarose ilaa 55°C kuna dar dheega ethidium bromide (EB) dheega ugu dambeeya ee 0.5 μg/ml.
(c) Ku xidh cidhifyada dhalooyinka ama saxannada akriliga leh qadar yar oo jel agarose ah, ku dar shanlo, oo dhig ilkaha shanlada ilaa 0.5 ~ 1.0 mm ka sarreeya saxanka.
(d) Si joogto ah ugu shub xalalka agarose jelka dhalaalay si joogto ah dhalada ama saxan akrilik ah (dhumucdu waxay ku xidhan tahay mugga muunadda DNA-da), adigoo iska ilaalinaya hordhaca goobooyinka hawada. Si dabiici ah ha u adkeyso heerkulka qolka.
(e) Si taxadar leh uga saar shanlada ka dib markaad si buuxda u adkeyso. Ku dar qadar habboon oo ah qalabka elektrophoresis taangiga jel, iyadoo la hubinayo in saxanka jelka uu hoos ugu jiro 1 mm ka hooseeya dusha sare ee qalabka elektrophoresis.
(2) Diyaarinta Gel Agaroose Toosan
(a) Ka saar dufan ama hadhaaga taarikada dhalada adigoo ku dhaqaya ethanol.
(b) Dhig taarikada meelaha bannaan ee u dhexeeya biyo-xireennada hore iyo dambe, ku toosi cidhifyada taarikada bannaanka iyo hore iyo dambe, oo ku xidh xidhyo.
(c) Ku dar 2% agarose ku jira 1x meel u dhaxaysa cidhifyada taarikada meelaha bannaan si aad u samaysato 1 sentimitir agarose-sare xagga hoose ee qolka shubka jeelka.
(d) Ku shub jelka agarose-ka ee dhalaaliyay fiirsiga la rabo, oo lagu diyaariyey 1x baffer, geli qolka jelka ilaa 1 cm ka hooseeya sare.
(e) Geli shanlada, adiga oo iska ilaalinaya in ay ku dabin goobooyin hawo hoostooda ilkaha shanlada. Mararka qaarkood, laalaabku waxay ka soo muuqan karaan ilkaha shanlada inta lagu jiro qaboojinta jel agarose; xaaladahan oo kale, si fudud ku dar qaar ka mid ah agarose dhalaalay xagga sare si aad u xoojiso.
(f) Ka saar shanlada. Si aad uga hortagto daadinta bakhaarka booska rarka, xidh xidhiidhka ka dhexeeya saxanka jelka agarose iyo qolka elektrophoresis 2% agarose oo ku dar qaddarka kaydinta loo baahan yahay.
(g) Ku dar 1x electrophoresis baffer qolka jelka.
(h) Si taxadar leh ugu shub shaybaarka DNA-ga ku shub jelka agarose ee ka hooseeya kaydka.
Macluumaad dheeraad ah oo ku saabsan aqoonta aasaasiga ah ee ku saabsan agarose gel electrophoresis, waxaanu wadaagi doonaa usbuuca soo socda. U roon in macluumaadkani uu noqdo mid waxtar u leh tijaabadaada.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ayaa ku takhasusay soo saarista qalabka electrophoresis in ka badan 50 sano oo leh kooxdayada farsamada xirfadeed u gaar ah iyo xarunta R&D. Waxaan haysanaa khad wax soo saar oo la isku halayn karo oo dhamaystiran laga bilaabo naqshadaynta ilaa kormeerka, iyo bakhaar, iyo sidoo kale taageerada suuqgeynta. Alaabadayada ugu muhiimsan waa Electrophoresis Cell (taangi / qolka), Korontada Electrophoresis, Transilluminator Blue LED, Transilluminator UV, Muuqaalka Gel & Nidaamka Falanqaynta iwm.
Waxaan hadda raadineynaa la-hawlgalayaal, labadaba OEM electrophoresis taangiga iyo qaybiyeyaasha waa la soo dhaweynayaa.
Haddii aad leedahay wax qorshe ah oo wax iibsiga ah oo alaabtayada ah, fadlan ha ka waaban inaad nala soo xidhiidho. Waxaad fariin noogu soo diri kartaa iimaylka[email protected]ama[email protected], ama fadlan naga soo wac +86 15810650221 ama ku dar Whatsapp +86 15810650221, ama Wechat: 15810650221.
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Waqtiga post: Dec-07-2023