Tusaalaha Diyaarinta iyoSoodejinaya
Sababo la xiriira isticmaalka nidaamka difaaca joogtada ah iyada oo aan la isku dhejin jel inta badan, shaybaaradu waa inay lahaadaan feejignaan ku habboon iyo mug yar. Isticmaal apipettein si tartiib ah loogu daro muunada, oo leh 5-10 μg halkii ceel, si looga fogaado hoos u dhac weyn oo ku yimaada xallinta. Goormamuunad loading, korontada waa in la demiyaa. Muunadu waa inay ka kooban tahay dheeha dheeha (0.025% bromophenol blue or orange) iyo sukrose (10-15%) ama glycerol (5-10%) si loo kordhiyo cufnaanta, xooga saaro muunada, loogana hortago faafitaanka. Si kastaba ha noqotee, mararka qaarkood sukrose ama glycerol waxay sababi karaan xargaha U-qaabeeya natiijooyinka electrophoresis, taas oo laga fogaan karo iyadoo la adeegsanayo 2.5% Ficoll (polyvinylpyrrolidone).
Electrophoresis
Danabku waa 5-15 V/cm, guud ahaan qiyaastii 10 V/cm. Kala soocida molecules waaweyn, danabku waa inuu ahaadaa mid hooseeya, inta badana aan ka badnayn 5 V / cm.
wasakhaynta
Dheecaan fluorescent ethidium bromide (EB) ayaa caadi ahaan loo isticmaalaa wasakhaynta si loo eego xargaha DNA-da ee gel agarose. EB waxay geli kartaa inta u dhaxaysa lammaane saldhig oo DNA ah, taasoo keenaysa EB inay ku xidho DNA-da. Nuugista iftiinka ultraviolet ee 260 nm ee DNA waxaa loo wareejiyaa EB, EB-xiran wuxuu sii daayaa fluorescence at 590 nm gobolka casaan-orange ee iftiinka muuqda. Ku wasakhaynta jelka ee 1 mmol/L MgSO4 xal 1 saac ah waxay yarayn kartaa fluorescence-ka asalka ah ee ay keento EB-ga aan xidhnayn, fududaynta ogaanshaha xaddi yar oo DNA ah.
Midabka EB wuxuu leeyahay faa'iidooyin dhowr ah: way fududahay in la isticmaalo, ma jebiyo asiidhyada nucleic, waxay leedahay dareen sare, waxayna wasakhayn kartaa labadaba DNA iyo RNA. EB waxaa lagu dari karaa muunada waxaana lala socon karaa iyadoo la isticmaalayo nuugista UV wakhti kasta. Wasaaqaynta ka dib, EB waxaa lagaga saari karaa marka la soo saaro n-butanol.
Si kastaba ha ahaatee, midabka EB waa mutagen awood leh, iyo taxadarka, sida xidhashada galoofyada polyetylen, waa in la qaadaa inta lagu jiro wax ka qabashada. Acridine orange sidoo kale waa dheeha caadiga ah ee la isticmaalo sababtoo ah waxay kala saari kartaa inta u dhaxaysa asiidhyada nucleic-ka ah ee hal-xadhigle iyo laba-jibbaaran (DNA, RNA). Waxay soo bandhigaysaa fluorescence cagaaran (530 nm) oo loogu talagalay asiidhyada nucleic-ka ah ee laba-jibbaaran iyo fluorescence cas (640 nm) oo loogu talagalay asiidhyada nucleic-ka ah ee hal-xidhaale leh. Intaa waxaa dheer, midabada kale sida buluugga methylene, cagaarka methylene, iyo quinoline B ayaa loo isticmaali karaa midabaynta.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ayaa ku takhasusay soo saarista qalabka electrophoresis in ka badan 50 sano oo leh kooxdayada farsamada xirfadeed u gaar ah iyo xarunta R&D. Waxaan haysanaa khad wax soo saar oo la isku halayn karo oo dhamaystiran laga bilaabo naqshadaynta ilaa kormeerka, iyo bakhaar, iyo sidoo kale taageerada suuqgeynta. Alaabadayada ugu muhiimsan waa Electrophoresis Cell (taangi / qolka), Korontada Electrophoresis, Transilluminator Blue LED, Transilluminator UV, Muuqaalka Gel & Nidaamka Falanqaynta iwm.
Waxaan hadda raadineynaa la-hawlgalayaal, labadaba OEM electrophoresis taangiga iyo qaybiyeyaasha waa la soo dhaweynayaa.
Haddii aad leedahay wax qorshe ah oo wax iibsiga ah oo alaabtayada ah, fadlan ha ka waaban inaad nala soo xidhiidho. Waxaad fariin noogu soo diri kartaa iimaylka[email protected]ama[email protected], ama fadlan naga soo wac +86 15810650221 ama ku dar Whatsapp +86 15810650221, ama Wechat: 15810650221.
Fadlan sawir koodka QR si aad ugu darto Whatsapp ama WeChat.
Waqtiga boostada: Dec-20-2023