Cellulose Acetate Membrane Electrophoresis

Waa maxay Cellulose Acetate Membrane Electrophoresis?

Cellulose Acetate Membrane Electrophoresis waa mid ka mid ah farsamooyinka electrophoresis ee isticmaala xuubabka acetate cellulose sida warbaahinta taageerta ee tijaabooyinka.

Cellulose Acetate waa nooc ka mid ah acetate of cellulose kaas oo acetylated ka hydroxyl cellulose. Marka ay ku milmo dareeraha dabiiciga ah sida acetone, waxaa lagu dabooli karaa filim isku mid ah oo wanaagsan oo leh daloolo yaryar. Dhumucdiisuna waxay ku dhowdahay 0.1-0.15mm oo ku habboon tijaabada.

Faa'iidooyinka xuubka acetate cellulose

Marka la barbar dhigo warqadda fliter-ka, waxay leedahay faa'iidooyinka soo socda:

1.Saamaynta wanaagsan ee kala-tagga. Shaybaarada borotiinka, xuubka acetate cellulose ayaa nuugaya qaar ka mid ah, dheesha ka dib, waxay gebi ahaanba ka soomi kartaa midabka asalka ah. Xadhkaha dheehku aad bay u cad yihiin, sidaa awgeed waxay kor u qaadaysaa saxnaanta go'aaminta tirada.
2.Fast oo waqti badbaadi. Xuubka acetate cellulose wuu ka yar yahay hydyophilic marka loo eego xaashida fliter-ka, markaa waxqabadka korantada-osmosisku wuu ka yar yahay warqad fliter ah, korantada waxaa inta badan lagu sameeyaa muunado soo gaabin kara wakhtiga kala-goynta oo ka yarayn kara wakhtiga electrophoresis. Caadi ahaan, waqtiga korantada xuubka acetate cellulose waa qiyaastii 45-60 daqiiqo. Geedi socodka oo dhan ee electrophoresis oo ay ku jiraan midabaynta iyo midabaynta kaliya waxay u baahan yihiin 90 daqiiqo.
3.Dareen sare iyo muunado yar oo la isticmaalo. Waxay u baahan tahay kaliya 2 μL serum ee borotiinka serum electrophoresis.
Codsiga 4.Wide. Qaar ka mid ah borotiinka ma fududa in lagu kala saaro electrophoresis warqad, sida Alpha-fetoprotein, bacteriolytic enzyme, insulin, iyo histone iwm. Laakiin waxaa aad u fiican in la isticmaalo cellulose acetate xuubka electrophoresis si loo kala saaro iyaga.
5.Si fudud loo xafido iyo qiyaasid. Ka dib markaad dheehdo shaybaarada xuubka acetate cellulose acetate, muunada ku dhex geli xalka isku dhafan ee glacial acetic acid iyo ethanol si aad u samayso saxan qalalan oo hufan oo la keydiyo muddo dheer.

Sababtoo ah xuubka acetate cellulose electrophoresis waa sahlan tahay in la shaqeeyo, hadda waxaa si ballaaran loo isticmaalaa falanqaynta iyo tijaabinta borotiinka plasma, lipoprotein, glycoprotein, Alpha-fetoprotein, enzyme, polypeptide, nucleic acid iyo biomacromolecule kale.

Diyaarinta Cellulose Acetate Membrane Electrophoresis

Qalabka la isticmaalo waxaa ka mid ah koronto yar oo koronto ah, taangiga elektrophoresis jiifka ah. Warbaahinta taageerta waa xuubka acetate cellulose.

Reagen-yaasha:

1.pH8.6 xal barbitol ah: Diethyl barbituric acid, Diethyl sodium pentobarbital;
2.Stain: Ponceau S, Trichloroacetic;
3.TBS/T ama PBS/T: 95% ethanol, glacial acetic acid;
4.Cleaning Solution: ethanol anhydrous, glacial acetic acid.

Beijing Liuyi Biotechnology Co., Ltd waxay leedahay nidaam qaan-gaar ah oo loogu talagalay elektrophoresis xuubka acetate cellulose. Qaabka DYCP-38C waa qol jilicsan oo loogu talagalay waraaqaha iyo electrophoresis slide, iyo cellulose acetate electrophoresis. Jirka ugu weyn waa qaabaysan yahay, ma jirto ifafaale dareere ah. Qaabka korontada DYY-2C, DYY-6C, DAY-6D, DAY-8C, DAY-10C iyo DYY-12 ayaa ku siin kara koronto DYCP-38C. Guud ahaan, macmiilku waxa uu jecel yahay in uu u doorto model DYY-6C si uu koronto ugu noqdo DYCP-38C.

Sida badeecada lagama maarmaanka u ah taangiga elektrophoresis DYCP-38C, Liuyi wuxuu kaloo bixiyaa xuubka acetate cellulose. Waxaa jira saddex cabbir oo caadi ah oo xuubka ah, 7 * 9cm, 2 * 8cm iyo 12 * 8cm, waxaanan ku siin karnaa cabbirka xuubka acetate cellulose sidoo kale shuruudahaaga.

Qoraalada ku saabsan tijaabada:

1. Ku shub muunadda dhinaca qallafsan: 1-2UL waa ku habboon tahay
2.Electrophoresis joogta ah: xoogga hadda 0.4 ~ 0.6m A/cm
3.pH8.6 barbitol bakhtiiye xalinta: xoogga Ionic 0.06
4.Waqtiga midabaynta: 5 daqiiqo oo ku filan
5.Preservation: ku rid khariidadda elecrtophoresis engegan ee nadiifinta 10-15 daqiiqo, ka dibna ka soo bixi oo ku dheji galaas nadiif ah, ka dib marka ay qallajiso, waxay noqon doontaa khariidad filim oo hufan.

Calaamadda Liuyi waxay leedahay taariikh ka badan 50 sano oo Shiinaha ah waxayna shirkaddu ku siin kartaa alaab xasilloon oo tayo sare leh oo dhan adduunka oo dhan. Sannadaha horumarka, waxay u qalantaa doorashadaada!

Wixii macluumaad dheeraad ah oo nagu saabsan, fadlan nagala soo xiriir email ahaan[email protected], [email protected].