Gel electrophoresis waa mid ka mid ah hababka ugu waaweyn ee loo isticmaalo bayoolaji molecular ee falanqaynta DNA. Habkani wuxuu ku lug leeyahay guuritaanka jajabyada DNA-da iyada oo loo marayo jel, halkaas oo ay ku kala soocaan iyada oo ku saleysan cabbirka ama qaabka. Si kastaba ha ahaatee, waligaa ma la kulantay wax khalad ah inta lagu guda jiro tijaabooyinkaaga elektiroonigga ah, sida xargaha la isku mariyo ee jelka agarose, ama aan lahayn wax xirmo ah jel? Maxaa sabab u noqon kara khaladaadkan?
Farsamayaqaanadeenu waxay halkan ku soo koobeen lamaanayaasha cilad-raadinta si aad u tixraacdo.
1. Xadhkaha la isku marriyo ee jel agarose ah
●DNA-da ayaa hoos loo dhigay. Ka fogow faddaraynta nukliyeerka.
● Qalabka elektrophoresis-ka maaha mid cusub. Ka dib isticmaalka soo noqnoqda ee qalabka elektiroonigga ah, xoogga ionic ayaa hoos u dhacaya, qiimaha pH-ga ayaa kor u kacaya, markaa awoodda wax-qabadku way daciiftaa, taas oo saameynaysa saameynta electrophoresis. Waxaa lagu talinayaa in la beddelo qalabka elektrophoresis si joogto ah.
● Xaaladaha korantada ee aan habboonayn ayaa la isticmaalay. Ha u ogolaan danabku inuu dhaafo 20 V/cm, oo ilaali heerkulka <30°C inta lagu jiro electrophoresis. Xadhkaha DNA-ga weyn ee electrophoresis, heerkulku waa inuu ahaadaa <15° C. Hubi in bakhaarku leeyahay awood ku filan.
●Din aad u badan ayaa lagu shubay jelka. Yaree tirada DNA-ga.
● Milix badan oo ku jira DNA-da. Isticmaal roobabka ethanol si aad uga saarto milixda xad dhaafka ah ee horumarsan.
DNA-da waxaa ku wasakhoobay borotiin. Isticmaal soosaarida phenol si aad uga saarto borotiinka horumarsan.
● DNA-da waa la diiday. Ha kuleyl ka hor electrophoresis. Ku qas DNA-ga kaydka 20mM NaCl.
2. Anomalies guuritaanka band DNA
● Dib u habeynta goobta COS ee jajabka Hind III. Kuleylka DNA-da 5 daqiiqo ka hooseeya 65 ° C ka hor electrophoresis, ka dibna ku qabooji cutubka barafka 5 daqiiqo.
● Xaaladaha korantada ee aan habboonayn ayaa la isticmaalay. Ha u ogolaan danabku inuu dhaafo 20 V/cm, oo ilaali heerkulka <30°C inta lagu jiro electrophoresis. Hubi in koronto-fayrasku uu leeyahay awood wax-is-dejiya oo ku filan.
● DNA-da waa la diiday. Ha kuleyl ka hor electrophoresis. Ku qas DNA-ga kaydka 20mM NaCl.
3. Daal ama aan lahayn xargaha DNA ee jelka agarose
● Waxaa jiray tiro ku filan ama uruurinta DNA ee ku raran jelka. Kordhi tirada DNA-ga. Polyacrylamide gel electrophoresis wax yar ayuu ka xasaasisan yahay agarose electrophoresis, iyo rarida muunada si habboon ayaa loo dhimi karaa.
● DNA-da ayaa hoos u dhacday. Ka fogow faddaraynta nukliyeerka.
● DNA-da waxaa laga saaray jel-ka. Electrophorese jel waqti yar, isticmaal danab hoose, ama isticmaal jel boqolkiiba sare ah.
● Isha iftiinka W aan habboonayn ayaa loo isticmaalay aragtida DNA-da-dheellida-ethidium bromide. Isticmaal mowjadda gaaban (254 nm) iftiinka W si aad u dareento.
4. Xadhkaha DNA oo maqan
●Cabbirka yar ee DNA-da ayaa laga saaray jel-ka. Electrophorese jel waqti yar, isticmaal danab hoose, ama isticmaal jel boqolkiiba sare ah.
● Way adag tahay in la kala saaro xadhkaha DNA-da ee unugyada isku midka ah. Kordhi wakhtiga electrophoresis, oo hubi feejignaantaee jel si loo hubiyo in si sax ah boqolkiiba jel in la isticmaalo.
● DNA-da waa la diiday. Ha kuleyl ka hor electrophoresis. Ku qas DNA-ga kaydka 20mM NaCl.
Xadhkaha DNA-du aad bay u weyn yihiin, jel-ka-caadiga ahina kuma habboona. Falanqaynta garaaca wadnaha jel electrophoresis.Waa maxay arrimaha kale ee aad la kulantay agarose gel electrophoresis? Waxaan baari doonaa wax badan hagaha mustaqbalka.
Beijing Liuyi Biotechnology Co., Ltd (Liuyi Biotech) waa shirkad khaas ah oo diiradda saareysa alaabada la xidhiidha elektiroonigga ee Shiinaha. Sheekadeedu waxay bilaabantay 1970 markii Shiinuhu aanu wali gelin dib u habaynta iyo wakhtiga furitaanka. Intii lagu guda jiray horumarinta sano, Liuyi Bitotech waxay leedahay astaan u gaar ah, kaas oo loo yaqaan brand Liuyi ee suuqa gudaha ee alaabta electrophoresis.
Astaanta Liuyi waxay Shiinaha ku leedahay in ka badan 50-sano oo taariikh ah shirkaduna waxay ku siin kartaa alaab deggan oo tayo sare leh adduunka oo dhan. Inta lagu jiro sannadaha 'horumarinta, waa u qalantaa doorashadaada!
Unugyada electrophoresis jiifka ah (taangiyada / qolalka) ee Liuyi Biotech waa tayo sare oo muuqaal wanaagsan leh. Iyada oo cabbirrada kala duwan ee saxarada jel, waxay buuxin karaan shuruudahaaga tijaabada ah ee kala duwan. Waxaan leenahay kooxdayada farsamada iyo warshad noo gaar ah. Laga soo bilaabo naqshadeynta ilaa wax soo saarka, alaabta ceeriin ilaa qaybaha muhiimka ah, waxaan xakameyn karnaa habka oo dhan. Taxanaha DYCP 31 waxaa loogu talagalay electrophoresis DNA, kuwaas oo ah moodelDYCP-31BN, DYCP-31CN,DYCP-31DNiyoDYCP-31E. Farqiga u dhexeeya iyaga waa cabbirka jel iyo qiimaha. Waxaan siinaa cabbir buuxa oo alaab ah macaamiisheena. QaabkaDYCP-32Cwaxay samayn kartaa jel ugu weyn 250mm*250mm.
Dhanka kale, waxaan kugula talineynaa sahayda korantada ee electrophoresisDHAQAN-6C,DHAQAN-6DiyoDHAQAN-10Cunugyadeena electrophoresis (taangiyada/qolalka) DYCP-31 iyo 32 taxane.
Haddii aad rabto macluumaad dheeraad ah oo ku saabsan alaabta, fadlan booqo website-kan si aad u hesho wax badan, oo soo dhowow in aad nala soo xiriir email ahaan si aan u ogaano waxaad rabto, oo arag haddii aan ku siin karno xalalka adiga.
Wixii macluumaad dheeraad ah oo nagu saabsan, fadlan nagala soo xiriir email ahaan[email protected], [email protected].
Waqtiga boostada: Meey-09-2022