Mabda'a Tijaabada
Hemoglobin electrophoresis ujeedadiisu tahay in la ogaado oo la xaqiijiyo hemoglobins kala duwan oo caadi ah iyo kuwa aan caadi ahayn.
Sababo la xiriira khidmadaha kala duwan iyo dhibcaha isoelectric ee noocyada kala duwan ee hemoglobin, xal pH ah oo gaar ah, marka barta isoelectric ee haemoglobin ay ka hooseyso pH ee xalinta, hemoglobin wuxuu qaadaa lacag taban wuxuuna u guuraa anode inta lagu jiro electrophoresis. Taa beddelkeeda, hemoglobin oo leh lacag togan ayaa u dhaqaaqday dhinaca cathode.
Marka la eego danab gaar ah iyo ka dib wakhti gaar ah oo elektrophoresis ah, hemoglobins leh kharashyo kala duwan iyo miisaan kelliyadeed ayaa soo bandhigaya jihooyin iyo xawliyo kala duwan oo socdaalka ah. Tani waxay u ogolaaneysaa kala soocida aagagga kala duwan, ka dibna falanqaynta midabka midabka ama korantada ayaa lagu samayn karaa aagaggaas si loo qiyaaso hemoglobins kala duwan. Habka ugu badan ee la isticmaalo waa pH 8.6 cellulose acetate xuubka electrophoresis.
Sitobalaasamka dhexdiisa, kooxaha ethylene glycol (CHOH-CHOH) ee ku jira glycogen ama maaddooyinka polysaccharide (sida mucopolysaccharides, mucoproteins, glycoproteins, glycolipids, iwm.) ayaa oksaydhaysan by periodic acid oo loo beddelo kooxaha aldehyde (CHO-CHO). Kooxahan aldehyde waxay ku daraan reagent Schiff-cas oo aan midab lahayn, samaynta dheeh guduud-guduudan oo dhigata halka polysaccharides ay ku jiraan unugga. Dareen-celintan waxa loo yaqaannaa wasakhaynta aashitada-Schiff (PAS), oo hore loogu tilmaami jiray midabaynta glycogen.
Habka Tijaabada
Qalabka:Cellulose acetatemembrane, qalabka electrophoresis(DYCP-38C iyo korontada DYY-6C), Qalabka Soodejinta Tusaalaha Sarepipette), spectrophotometer, cuvettes midab leh, kaydiyayaal.
kaydinta:
(1) pH 8.6 TEB Buffer: Miisaanka 10.29 g Tris, 0.6 g EDTA, 3.2 g boric acid, oo ku dar biyo la miiray ilaa 1000 ml.
(2) Borate Buffer: Miisaanka 6.87 g borax iyo 5.56 g boric acid, oo ku dar biyo la shiiday 1000 ml.
Habka:
Pdib u hagaajinta Hemoglobin Solution
Qaado 3 ml oo dhiig ah oo uu ku jiro heparin ama sodium citrate sida xinjirowga lidka ku ah. Centrifuge at 2000 rpm 10 daqiiqo oo iska tuur balaasmaha. Unugyada dhiigga cas saddex jeer ku dhaq cusbo nafsiyeed (750 rpm, 5 daqiiqo sentrifugation mar kasta). Centrifuge at 2200 rpm 10 daqiiqo oo iska tuur dusha sare. Ku dar qadar isku mid ah oo biyo nadiif ah, ka dibna ku dar 0.5 jeer mugga tetrachloride kaarboon. Si xoog leh u rux ilaa 5 daqiiqo, ka dibna sentrifuge at 2200 rpm 10 daqiiqo si aad u ururiso xal Hb sare si loo isticmaalo dambe.
Qooynta Xubinta
Iska yaree xuubka acetate cellulose xariijimo cabbiraya 3 cm × 8 cm. Ku qooy pH 8.6 TEB baffer ilaa ay si buuxda u buuxsamaan, ka dibna ka saar oo ku qallaji warqad filter ah.
Bararka
Isticmaal pipette si aad u ogaatid 10 μl ee xalka haemoglobin si toos ah ugu dheji xuubka acetate cellulose (dhinaca qallafsan), oo ku saabsan 1.5 cm cidhifka.
Electrophoresis
Ku shub xalka borate baffer qolka electrophoresis. Dhig xuubka acetate cellulose oo leh dhinaca la arkay ee dhamaadka cathode ee qolka. Ku wad 200 V 30 daqiiqo.
Ilbaxnimo
Ka jar aagagga HbA iyo HbA2, ku rid tuubooyin tijaabo oo kala duwan, kuna dar 15 ml iyo 3 ml oo biyo nadiif ah, siday u kala horreeyaan. Si tartiib ah u rux si aad gabi ahaanba uga saarto haemoglobin-ka, ka dibna isku qas.
Midabka midabka
eber nuugista iyadoo la isticmaalayo biyo la miiray si loo xalliyo oo cabbir nuugista 415 nm.
Xisaabinta
HbA2(%) = nuugista tuubada HbA2 / ( nuugista tuubada HbA2 × 5 + nuugista tuubada HbA2) × 100%
Xisaabinta Natiijooyinka Tijaabada
Qiyaasta Tixraaca pH 8.6 TEB Buffer Cellulose Acetate Electrophoresis: HbA> 95%, HbA2 1%-3.1%
Xusuusin
Waqtiga Electrophoresis waa inuusan noqon mid aad u dheer. Xuubka acetate cellulose waa inuusan engegin inta lagu jiro electrophoresis. Jooji electrophoresis marka HbA iyo HbA2 ay si cad u kala soocaan. Electrophoresis-da dheer waxay sababi kartaa faafin iyo blur.
Iska ilaali isticmaalka muunad aad u badan. Dareeraha hemoglobin-ka ee xad-dhaafka ah waxay u horseedi kartaa kala go'a bandowga ama midab-ku-filan, taasoo keenta heerar been abuur ah oo HbA ah.
Kahortagga wasakheynta xuubka acetate cellulose oo leh borotiinno.
Hadda waa inuusan aad u sarreyn; Haddii kale, xadhkaha hemoglobin ma kala sooci karaan.
Had iyo jeer ku dar shaybaarada shakhsiyaadka caadiga ah iyo hemoglobin-yada aan caadiga ahayn ee lagama maarmaanka u ah sida kontaroolada.
Beijing Liuyi Biotechnology waxay soo saartaa taangiga elektrophoresis ee xirfadlaha ah ee hemoglobin electrophoresis kaas oo ah moodelkaDYCP-38Ccellulose acetate xuubka electrophoresis taangiga, waxaana jira laba nooc oo koronto koronto ah oo diyaar u ah taangiga xuubka acetate celluloseDHAQAN-2CiyoDHAQAN-6Ckorontada.
Dhanka kale, Beijing Liuyi Biotechnology waxay siisaa macaamiisha xuubka acetate cellulose, iyo cabbirka xuubka acetate cellulose waa la habeyn karaa. Ku soo dhawoow inaad na weydiisato muunado iyo macluumaad dheeraad ah.
Astaanta Beijing Liuyi waxay leedahay in ka badan 50-sano oo taariikh ah Shiinaha shirkadduna waxay ku siin kartaa alaab deggan oo tayo sare leh adduunka oo dhan. Inta lagu jiro sannadaha horumarinta, waxay u qalantaa doorashadaada!
Waxaan hadda raadineynaa la-hawlgalayaal, labadaba OEM electrophoresis taangiga iyo qaybiyeyaasha waa la soo dhaweynayaa.
Haddii aad leedahay wax qorshe ah oo wax iibsiga ah oo alaabtayada ah, fadlan ha ka waaban inaad nala soo xidhiidho. Waxaad fariin noogu soo diri kartaa iimaylka[email protected]ama[email protected], ama fadlan naga soo wac +86 15810650221 ama ku dar Whatsapp +86 15810650221, ama Wechat: 15810650221
Waqtiga boostada: Seb-20-2023