Agakacaygel electrophoresis
Agarose gel electrophoresis waa habka jel electrophoresis ee loo isticmaalo biochemistry, bayoloji molecular, genetics, iyo kimistari kiliinikada si loo kala saaro dadka isku dhafan ee macromolecules sida DNA amaRNA.Tani waxa lagu gaaraa in la dhaqaajiyo unugyaraha aashitada nucleic-ka ah ee si xun loo dallaco iyada oo loo marayo matrix agarose lehelectrophoresis.
Qalabka agarose jel
Agarose jel waa matrix saddex-geesood ah oo ka samaysan molecules agarose helical oo ku jira xidhmooyin supercoiled kuwaas oo la isku daray qaab dhismeedka saddex-geesoodka ah ee kanaalka iyo daloolada kuwaas oo biomolecules ay mari karaan. Qaab dhismeedka 3-D waxaa si wadajir ah loo qabtaa bonds hydrogen oo sidaas darteed waa la carqaladayn karaa adoo ku celinaya xaalad dareere ah. Heerkulka dhalaalku wuu ka duwan yahay heerkulka gelling-ka, iyadoo ku xiran ilaha, agarose gelku wuxuu leeyahay heerkulbeeg heerkulkiisu yahay 35-42 °C iyo kulaylka dhalaalay 85-95 °C Agaroses-ku-dhalan-yar oo-gelling-yar oo lagu sameeyay wax-ka-beddel kiimikaad ayaa sidoo kale la heli karaa.
Faa'iidooyinka iyo khasaaraha ee agarose gel electrophoresis
Faa'iidada ugu weyn ee farsamada agarose gel electrophoresis waa in si fudud loo farsamayn karo iyo molecule DNA-ga loo isticmaalo muunad ahaan sidoo kale waa la soo ceshan karaa iyada oo aan waxyeello loo geysan dhamaadka habka. Sababtoo ah jel agarose ma sameeyo't ka kooban sulfate iyo kooxda karboksyl, ma keeni doonto electroosmosis ee agar. Iyada oo qaab-dhismeedka labbiska ah iyo cabbirka daloolka weyn, jelka agarose wuxuu u fiican yahaykala soocida unugyo DNA oo waaweyn, sida enzyme adag, nucleic acid iyo fayraska. Agarose gel ma sameeyo't nuugo iftiinka UV, sidaas darteed si toos ah ayaa loo wasakheeyaa ama ka dib marka la gooyo si ay u noqoto filim, sidoo kale waa la bedeli karaa.
Waxa kale oo jira qaar ka mid ah faa'iido darrada farsamada agarose gel electrophoresis. Agarose gel si fudud ayaa loo jabiyaa, sidaas darteed cufnaanta ma noqon karto mid aad u hooseeya, waxay ku kici doontaa agarose badan.Gel-garooska Way adag tahay in la badbaadiyo sababtoo ah way fududahay in ay wasakhayso bakteeriyada, waana in la diyaariyaa ka hor inta aan la isticmaalin. Marka la barbar dhigo farsamada PAGE electrophoresis, waxtarka shaandhada molecular waa ka yar tahay, iyo aagga electrophoresis waa ka yar yahay.
Horizontalelectrophoresishabka loogu talagalay gel agarose
Marka laga reebo agarose iyo buffer, agarose gel electrophoresis tijaabadu waxay u baahan tahay nidaamka electrophoresis siman iyo hoose-korantada korantada ee electrophoresis.Bejing Liuyi Biotechnology waxay bixin kartaa nidaamka elektrophoresis jiifka iyo Korontada electrophoresis, sida modelDYCP-31BN, DYCP-31CN waayonidaamka electrophoresis siman iyoDHAQAN-6C Korontada.
Waxay ku xiran tahay oo ku saabsan cabbirrada jel ee kala duwanlooga baahan yahay agrose gel electrophoresis tijaabo, cabbirrada kala duwan ee nidaamka electrophoresis siman waxaa bixin kara by Beijing Liuyi Biotechnology. Qaabka taxanaha ah ee DYCP-31,ssidaDYCP-31BN, DYCP-31CN, DYCP-31DN, DYCP-31E, DYCP-32BiyoDYCP-32CWaxay leeyihiin cabbirro kala duwan oo saxaarad jel ah, laga bilaabo tuurista jel cabbir yar ilaa jel cabbirka weyn, waxay la kulmi karaan tijaabadaadashuruudaha.
Calaamadda Liuyi waxay leedahay taariikh ka badan 50 sano oo Shiinaha ah waxayna shirkaddu ku siin kartaa alaab xasilloon oo tayo sare leh oo dhan adduunka oo dhan. Sannadaha horumarka, waxay u qalantaa doorashadaada!
Wixii macluumaad dheeraad ah oo nagu saabsan, fadlan nagala soo xiriir email ahaan[email protected], iibka01@ly.com.cn
Waqtiga boostada: Abriil-16-2022