Qaybta bayoolajiga molecular ee weligeed-soo-noqoshada ah, falcelinta silsiladda polymerase (PCR) iyo Gel Electrophoresis waxay u soo baxeen farsamooyin aasaasi ah oo fududeeya daraasadda iyo wax-is-dabamarinta DNA-da. Hababkani kaliya maaha kuwo udub dhexaad u ah cilmi-baarista laakiin sidoo kale waxay leeyihiin codsiyo baahsan oo ku saabsan ogaanshaha, sayniska dambi-baarista, iyo bayotechnoolajiyada.
Sawir laga soo qaaday mareegta biology4alevel
PCR waa farsamo kacaan oo ay samaysay Kary Mullis 1983kii, taas oo u ogolaanaysa saynisyahanada in ay si wayn u xoojiyaan qayb DNA ah oo gaar ah. Nidaamku wuxuu ku bilaabmaa kala-baxa DNA-da, halkaas oo DNA-da laba-geesoodka ah lagu kululeeyo ilaa 94 ° C, taas oo keenaysa inay u kala soocdo laba xargo oo keliya. Tan waxaa ku raaca soo-jiidashada, halkaas oo asal-taxanayaal-gaaban ee nucleotide-ku xidhaan taxanaha is-kaabaya ee DNA-da hal-xidhaale leh heerkul hoose (badanaa ku dhawaad 55°C). Ugu dambeyntii, marxaladda fidintu waxay ku dhacdaa 72 ° C, halkaas oo enzyme DNA polymerase uu ku dhejiyo qayb cusub oo DNA ah isagoo ku daraya nucleotides-yada asaasiga ah. Wareeggani waxa lagu soo noqnoqda 20-40 jeer, taasoo horseedaysa malaayiin nuqul oo isku xigxiga DNA-da.
Mashiinka PCR ee Beijing LIUYI
Marka DNA-da la kordhiyo, Gel Electrophoresis waxaa loo shaqeeyaa si loo kala saaro loona falanqeeyo alaabta PCR. Farsamadani waxay ku lug leedahay guuritaanka jajabyada DNA-da iyada oo loo marayo matrix gel agarose oo hoos yimaada saamaynta koronto. Unugyada DNA-da ayaa si xun loo dallacay sababtoo ah laf-dhabarka fosfateetka, waxayna u guuraan dhanka korantada togan. Jeelku wuxuu u shaqeeyaa sidii shaandho, taas oo u oggolaanaysa jajabyada DNA-da yaryar inay ka dhaqso badan yihiin kuwa waaweyn. Natiijo ahaan, jajabyada DNA-da ayaa la kala soocaa iyadoo lagu saleynayo cabbirkooda, oo leh xargo kala duwan oo lagu arki karo iftiinka ultraviolet ka dib markii lagu dheehdo dheeha sida ethidium bromide.
Beijing LIUYIAlaabta Electrophoresis jel
Isku darka PCR iyo Gel Electrophoresis ayaa ku awood badan codsiyo badan. Baaritaannada caafimaadka, PCR waxaa loo isticmaalaa in lagu ogaado jiritaanka cudur-sidaha, isbeddellada hidde-sideyaasha, ama taxane DNA oo gaar ah oo la xiriira cudurka. Gel Electrophoresis ayaa markaa u oggolaanaya aragtida iyo xaqiijinta jajabyada DNA-da ee la xoojiyay. Sayniska dambi baarista, farsamooyinkan ayaa muhiim u ah faraha DNA, halkaas oo ay ka caawinayaan in la is waafajiyo muunadaha DNA-da ee goobaha dembiyada iyo kuwa tuhmanayaasha.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ayaa ku takhasusay soo saarista qalabka electrophoresis in ka badan 50 sano oo leh kooxdayada farsamada xirfadeed u gaar ah iyo xarunta R&D. Waxaan haysanaa khad wax soo saar oo la isku halayn karo oo dhamaystiran laga bilaabo naqshadaynta ilaa kormeerka, iyo bakhaar, iyo sidoo kale taageerada suuqgeynta. Alaabtayada ugu muhiimsan waa Electrophoresis Cell (taangi / qolka), Korontada Electrophoresis, Transilluminator Blue LED, UV Transilluminator, Gel Image & Nidaamka Falanqaynta iwm. Waxaan sidoo kale siinaa qalabka shaybaarka sida qalabka PCR, qasiyaha vortex iyo centrifuge ee shaybaarka.
Beijing LIUYIAlaabta Electrophoresis jel
Haddii aad leedahay wax qorshe ah oo wax iibsiga ah oo alaabtayada ah, fadlan ha ka waaban inaad nala soo xidhiidho. Waxaad fariin noogu soo diri kartaa iimaylka[email protected]ama[email protected], ama fadlan naga soo wac +86 15810650221 ama ku dar Whatsapp +86 15810650221, ama Wechat: 15810650221.
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Waqtiga boostada: Agoosto-15-2024