Diyaarinta Gel Agarose ee Electrophoresis

Diyaarinta Agarose Gel ee Electrophoresis

Xusuusin: Had iyo jeer xidho galoofyada la tuuri karo!

Tilmaamaha Tallaabo-tallaabo

Miisaanka Budada Agarose:uwarqad miisaamaya iyo dheelitirka elegtarooniga ah si loo cabbiro 0.3g oo budada agarose ah (oo ku salaysan nidaam 30ml ah).

Diyaarinta TBST Buffer:pdib u celi 30ml oo ah 1x TBST bakhaar 100ml Erlenmeyer.

Kala dirida budada Agaroose:pbudada agarose-ka ku shub weelka TBST oo si fiican u ruxGeli iyagaku rid microwave iyo kulayl (sida caadiga ah 50 ilbiriqsi, oo lagu daboolay bireed aluminium ah) ilaa si buuxda u milmay.

Qaboojinta iyo Ku darista Nukliyeerka:uGac- galoofyada si aad isku dhafka uga saartid mikrowaytka oo aad wax yar ku qaboojiso biyo qabow ilaa ay diirran tahay (qiyaastii 60°C). Ku dar 2µl oo nuclease ah (Beddelka Eb) intaad ruxayso si aad si fiican isugu walaaqdo.

Diyaarinta Caagagga Gel:

• Ccufan oo qalajisaxaarad jel ah iyo qalabka wax lagu shuboee taangiga electrophoresis.
•  Pshalash gelsaxaaradgeli haanta gudaha oo geli shanlada meel go'an.
• Isku qas xalalka agarose, oo la qaboojiyey ilaa 65 ° C, oo si taxadar leh ugu shubsaxaarad jelgudahaqalabka wax lagu shubo jel, si tartiib ah u faafi ilaa ay ka sameyso lakab xitaa jel ah oo ku yaal saxanka dhalada.
• U oggolow inuu ku fadhiisto heerkulka qolka ilaa uu jelku si buuxda u adkeeyo.
• Si tartiib ah uga saar shanlada si toos ah oo ka saar cajaladda.
• Gelida gelisaxaaradgalay taangiga electrophoresis.

Muhiim: Hubi in aysan jirin xumbo hawo aagga ilkaha shanlada. Dusha sare ee dareeruhu waa inuu ahaadaa mid siman oo aan lahayn doogyo.

Orodka Gel

Soodejinaya jeelka

Ka dib markii jelku adkeeyo, ku rid taangiga electrophoresis oo ku shub bakhaarka electrophoresis ilaa jelku hoos u dhaco.

Diyaarinta Tusaalooyinka

• Ka soo qaad calaamadeeyaha iyo kaydinta qaboojiyaha.
• Ku dar 6µl oo ah kaydka rarka shaybaarka oo si fiican isku walaaq.
• Adigoo isticmaalaya micropipette, si tartiib ah shaybaarada ugu shub ceelasha waaweyn ee jelka (ka taxadar inaadan daloolin jel-ka oo ha bixinin mugga oo dhan si aad uga fogaato xumbo hawo).
• Ku dheji calaamadeeyaha mid ka mid ah ceelasha yaryar (xusuusnow booskiisa).

Bilawga Electrophoresis

• Dabool taangiga electrophoresis oo billow electrophoresis isla markiiba ka dib marka la shubo jelka.
• U deji korantada ilaa 60-100V. Muundooyinku waxay ka guuri doonaan korantada taban (madow) una guuri doona elektrodeyga togan (casaan).
• Korontada sare waxay soo gaabinaysaa kala-soocidda waxtarka leh ee jel agarose.
• Jooji electrophoresis marka dheeha buluuga ah ee bromophenol uu gaaro ilaa 1cm cidhifka hoose ee saxanka jelka.

U fiirsashada Natiijooyinka

Ka dib marka la kala saaro xargaha, jooji electrophoresis, soo saar jel, oo si toos ah u ogow oo sawir.

Isticmaal habka sawir-qaadista jel si aad u sawirto oo aad u fiirsato xargaha (ka hubi haddii ay jiraan wax xargo ah calaamadeeyaha ama muunadaha).

Ka dib markaad hesho khariidadda band-gaaga, hel calaamadeeyaha. Iyada oo ku saleysan calaamadeeyaha, waxaad go'aamin kartaa kooxaha bartilmaameedka ah!

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ayaa la soo saaray alaabada electrophoresis in ka badan 50 sano. Waxaan halkan ku soo bandhigaynaa nidaamyada electrophoresis jiifka ahagarose jelelectrophoresis.Doorohababka electrophoresis simanlaga bilaabo Beijing liuyi ee tijaabada gel electrophoresis si ay kaaga caawiso cilmi baaristaada warshadaha bayotechnoolajiyada.

Waxaan bixinaa noocyo kala duwan oo ah taangiyada electrophoresis jiifka ah ee tuurista iyo socodsiinta yar yaragaroosejel si weynagaroosegeela

Xulashada Hagaha Horizontal immersed Gel Electrophoresis Unit

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ayaa ku takhasusay soo saarista qalabka electrophoresis in ka badan 50 sano oo leh kooxdayada farsamada xirfadeed u gaar ah iyo xarunta R&D. Waxaan haysanaa khad wax soo saar oo la isku halayn karo oo dhamaystiran laga bilaabo naqshadaynta ilaa kormeerka, iyo bakhaar, iyo sidoo kale taageerada suuqgeynta. Alaabtayada ugu muhiimsan waa Electrophoresis Cell (taangi / qolka), Korontada Electrophoresis, Transilluminator Blue LED, UV Transilluminator, Gel Image & Nidaamka Falanqaynta iwm. Waxaan sidoo kale siinaa qalabka shaybaarka sida qalabka PCR, qasiyaha vortex iyo centrifuge ee shaybaarka.

Haddii aad leedahay wax qorshe ah oo wax iibsiga ah oo alaabtayada ah, fadlan ha ka waaban inaad nala soo xidhiidho. Waxaad fariin noogu soo diri kartaa iimaylka[email protected]ama[email protected], ama fadlan naga soo wac +86 15810650221 ama ku dar Whatsapp +86 15810650221, ama Wechat: 15810650221.

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