Wax dhibaato ah miyaad kala kulantaa diyaarinta jel agarose?LasocoFarsamoyaqaankayaga shaybaadhka diyaarinta jeelka.
Habka diyaarinta ee gel agarose waxay ku lug leedahay tallaabooyinka soo socda:
Miisaanka Budada Agarose
U miisaami cadadka loo baahan yahay ee budada agarose si waafaqsan fiirsashada la rabo ee tijaabadaada. Heerarka agarose ee caadiga ah waxay u dhexeeyaan 0.5% ilaa 3%. Xoojin sare ayaa loo isticmaalaa in lagu kala saaro unugyo DNA oo yaryar, halka uruurinta hoose ay tahay molecules waaweyn.
Diyaarinta Xalka Buffer
Ku dar budada agarose meel ku haboon korantada, sida 1× TAE ama 1× TBE. Mugga kaydka waa inuu u dhigmaa mugga jel ee loo baahan yahay tijaabadaada.
Kala dirida Agarooska
Kuleyli agaroseka iyo isku darka baffer ilaa agaroosku si buuxda u milmo. Tan waxaa lagu samayn karaa microwave ama saxan kulul. Si tartiib ah u walaaq xalka si uusan u karkarin. Xalka agarose waa inuu noqdaa mid cad oo aan lahayn qaybo muuqda.
Qaboojinta Xalka Agaroose
U ogolow xalka agarose ee kulul inuu qaboojiyo ilaa 50-60 ° C. Walaaq xalka inta lagu jiro habka qaboojinta si aad uga hortagto adkeyn degdeg ah.
Ku darida Wasaqda Asiidhka Nucleic (Ikhtiyaar)
Haddii aad rabto inaad sawirto DNA ama RNA ee jel, waxaad ku dari kartaa wasakhda nucleic acid, sida GelRed ama ethidium bromide, marxaladan. Markaad la tacaalayso wasakhyadan, xidho galoofyada oo taxadar, waayo waxay noqon karaan sun.
Ku shubista Gel
Ku shub xalka agarose ee la qaboojiyey galay caaryada jel electrophoresis diyaarsan. Geli shanlada si aad u abuurto ceelal muunad ah, adoo hubinaya in shanladu ay tahay mid sugan oo xalka si siman loogu qaybiyay caaryada.
Adkeynta Gel
U ogolow jelku inuu ku adkeysto heerkulka qolka, kaas oo caadi ahaan qaata 20-30 daqiiqo iyadoo ku xidhan xoojinta iyo dhumucda jelka.
Rkicinta Comb
Marka jelku si buuxda u adkeysto, si taxadar leh uga saar shanlada si aad u muujiso ceelasha saamiga. Geli jelka oo ay weheliso caaryada gudaha qolka electrophoresis oo ku dabool qaddarka ku habboon ee qalabka elektiroonigga ah, hubinta in jelku si buuxda u liqo.
Diyaarinta Electrophoresis
Ka dib markii jelku diyaar yahay, ku shub muunadahaaga ceelasha, oo sii wad tijaabada elektrophoresis-ka.
Haddii aad qabto wax arrimo ah oo ku saabsan diyaarinta jel, xor u tahay inaad nala soo xiriirto. Waxaan haynaa farsamayaqaano shaybaadhka ah oo diyaar u ah inay kula soo xiriiraan.
Aad ayaan ugu faraxsanahay inaan wadaagno war wanaagsan: taangigayaga caanka ah ee DYCP-31DN taangiga elektrophoresis ee tooska ah ayaa hadda ku socda xayeysiis, nala soo xidhiidh wixii macluumaad dheeraad ah hadda!
DYCP-31DN taangiga electrophoresis jiifa
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ayaa ku takhasusay soo saarista qalabka electrophoresis in ka badan 50 sano oo leh kooxdayada farsamada xirfadeed u gaar ah iyo xarunta R&D. Waxaan haysanaa khad wax soo saar oo la isku halayn karo oo dhamaystiran laga bilaabo naqshadaynta ilaa kormeerka, iyo bakhaar, iyo sidoo kale taageerada suuqgeynta. Alaabtayada ugu muhiimsan waa Electrophoresis Cell (taangi / qolka), Korontada Electrophoresis, Transilluminator Blue LED, UV Transilluminator, Gel Image & Nidaamka Falanqaynta iwm. Waxaan sidoo kale siinaa qalabka shaybaarka sida qalabka PCR, qasiyaha vortex iyo centrifuge ee shaybaarka.
Haddii aad leedahay wax qorshe ah oo wax iibsiga ah oo alaabtayada ah, fadlan ha ka waaban inaad nala soo xidhiidho. Waxaad fariin noogu soo diri kartaa iimaylka[email protected]ama[email protected], ama fadlan naga soo wac +86 15810650221 ama ku dar Whatsapp +86 15810650221, ama Wechat: 15810650221.
Fadlan sawir koodka QR si aad ugu darto Whatsapp ama WeChat.
Waqtiga boostada: Oct-15-2024