Waa maxay habka kulaylka baaskiilka ee PCR?

Falcelinta silsiladda polymerase (PCR) waa farsamo bayooloji molecular oo loo isticmaalo in lagu kordhiyo jajabyada DNA-da gaarka ah. Waxa loo qaadan karaa nooc gaar ah oo DNA-da ku-noqoshada oo ka baxsan noolaha. Tilmaamaha ugu muhiimsan ee PCR waa awoodda ay u leedahay inay si weyn u kordhiso tirada DNA-da.

Dulmar guud ee wareegga falcelinta silsiladda polymerase

Sawirka Bioninja

Waa maxay habka kulaylka baaskiilka ee PCR?

PCR wuxuu ka kooban yahay saddex tallaabo oo aasaasi ah:

1. Denaturation of Template DNA: Qaabka DNA-da waxa lagu kululeeyaa ilaa 93°C muddo go'an, taas oo keenaysa in DNA-da laba-geesoodka ah ay u kala baxdo hal xadhig, iyada oo u diyaarinaysa xidhitaanka asaasiga ah ee wareegga falcelinta soo socota.

2. Annealing of Template DNA and Primers: Denaturation ka bacdi, heerkulka waxaa hoos loo dhigayaa ilaa 55°C, taas oo u ogolaanaysa furayaasha in ay ku xidhaan taxanaha isdhaxgalka ah ee DNA-da hal-xidhaale leh.

3. Kordhinta Primers: At 72°C, DNA template-primer complex waxay maraysaa kordhinta iyadoo la kaashanayo DNA polymerase (sida Taq DNA polymerase), iyadoo la isticmaalayo dNTPs substrates si ay u abuurto xadhig cusub oo dhamaystiran oo ku salaysan DNA-ga template.

Mashiinka PCR ee Beijing LIUYI

Ku celcelinta denaturation, ka saarista, iyo geedi socodka fidinta, waxaa la soo saarayaa "xirfadaha konserfatifka ah ee nuska ah" ayaa la soo saarayaa, kuwaas oo u adeegi kara qaab-dhismeedka wareegyada xiga. Wareeg kastaa wuxuu qaataa qiyaastii 2 ilaa 4 daqiiqo, taasoo u oggolaanaysa hidda-wadaha bartilmaameedka ah in la kordhiyo malaayiin jeer 2 ilaa 3 saacadood gudahood.

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ayaa ku takhasusay soo saarista qalabka electrophoresis in ka badan 50 sano oo leh kooxdayada farsamada xirfadeed u gaar ah iyo xarunta R&D. Waxaan haysanaa khad wax soo saar oo la isku halayn karo oo dhamaystiran laga bilaabo naqshadaynta ilaa kormeerka, iyo bakhaar, iyo sidoo kale taageerada suuqgeynta. Alaabtayada ugu muhiimsan waa Electrophoresis Cell (taangi / qolka), Korontada Electrophoresis, Transilluminator Blue LED, UV Transilluminator, Gel Image & Nidaamka Falanqaynta iwm. Waxaan sidoo kale siinaa qalabka shaybaarka sida qalabka PCR, qasiyaha vortex iyo centrifuge ee shaybaarka.

Haddii aad leedahay wax qorshe ah oo wax iibsiga ah oo alaabtayada ah, fadlan ha ka waaban inaad nala soo xidhiidho. Waxaad fariin noogu soo diri kartaa iimaylka[email protected]ama[email protected], ama fadlan naga soo wac +86 15810650221 ama ku dar Whatsapp +86 15810650221, ama Wechat: 15810650221.

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