Gel Electrophoresis oo la garaaco CHEF Mapper A4
Tilmaamaha
| Qaabka | CHEF Mapper A4 |
| Korantada Gradient | 0.5V/cm ilaa 9.6V/cm, lagu kordhiyay 0.1V/cm |
| Hadda ugu badan | 0.5A |
| Korontada ugu badan | 350V |
| Xagasha garaaca | 0-360° |
| Time Gradient | toosan |
| Waqtiga Bedelka | 50ms ilaa 18h |
| Waqtiga Orodka ugu badan | 999h |
| Tirada Electrodes | 24, si madax banaan loo xakameeyo |
| Kala duwanaanta heerkulka | 0℃ ilaa 50℃, cilad ogaansho <±0.5℃ |
Sharaxaada
Gel electrophoresis pulsed field (PFGE) waxa ay kala saartaa molecules DNA-ga iyada oo beddesha beerta korantada inta u dhaxaysa lamaanayaasha korantada ee ku jihaysan meelo kala duwan, taas oo keenaysa molecules DNA-ga, kuwaas oo noqon kara malaayiin lammaane sal ah oo dheer si ay dib ugu habeeyaan oo ay ugu guuraan daloolada agarose jel xawaare kala duwan.Waxay ku gaartaa xallinta sare gudaha xadkan waxaana inta badan loo adeegsadaa bayoolajiga synthetic;aqoonsiga nasabka noolaha iyo microbial;cilmi-baaris ku saabsan epidemiology molecular;daraasadaha jajabyada plasmid ee waaweyn;meelaynta hiddo-wadaha cudurrada;khariidaynta jireed ee hiddo-wadaha, falanqaynta RFLP, iyo faraha DNA;cilmi-baarista dhimashada unugyada barnaamijka;daraasaadka ku saabsan dhaawaca iyo hagaajinta DNA;go'doominta iyo falanqaynta DNA-da genomic;kala soocidda koromosoomyada DNA;dhismaha, aqoonsiga, iyo falanqaynta ee maktabadaha genomic-jajabiyey ee waaweyn;iyo cilmi-baadhista transgenic.t uruurinta sida ugu hooseysa 0.5 ng/µL (dsDNA).
Codsiga
Ku habboon ogaanshaha iyo kala-soocidda molecules DNA ee u dhexeeya 100bp ilaa 10Mb cabbir ahaan, gaaritaanka xallinta sare ee xadkan.
Muuqaal
• Tignoolajiyada Sare: Waxay isku daraysaa CHEF iyo PACE tignoolajiyada goobta garaaca wadnaha si loo gaaro natiijooyin wanaagsan oo leh haad toosan oo aan leexleexanayn.
• Xakamaynta Madaxbanaan: Sifooyin 24 koronto oo platinum ah oo si madaxbanaan loo kontoroolo (dhexroor 0.5mm), iyadoo koronto kasta si gaar ah loo bedeli karo.
• Shaqada Xisaabinta Tooska ah: Isku-dubarid doorsoomayaal badan oo muhiim ah sida jaanis korantada, heerkulka, xagasha wareejinta, wakhtiga bilowga, wakhtiga dhamaadka, wakhtiga beddelka, wadarta wakhtiga orodka, danabka, iyo hadda xisaabinta tooska ah, ka caawinta isticmaalayaasha inay gaadhaan xaaladaha tijaabada ah ee ugu fiican.
Algorithm Gaarka ah: Wuxuu adeegsadaa algorithm kantaroolka garaaca garaaca gaarka ah si loo helo saameyn wanaagsan oo kala soocida, si fudud u kala saarta DNA-da toosan iyo wareega, iyadoo la xoojiyey kala-soocidda DNA-da wareeg ee ballaaran.
• Automation: Si otomaatig ah ayuu u diiwaangeliyaa oo dib u bilaabaa electrophoresis haddii nidaamka uu go'o koronto la'aan awgeed.
• Isticmaalaha-La Isku-habayn karo: Waxay u ogolaataa isticmaalayaasha inay dejiyaan shuruudahooda.
• Dabacsanaan: Nidaamku waxa uu dooran karaa jaranjarooyinka korantada ee gaarka ah iyo waqtiyada beddelka ee kala duwanaanshaha cabbirka DNA-ga gaarka ah.
• Shaashadda Weyn: Waxaa lagu qalabeeyay shaashad 7-inji ah oo LCD ah oo loogu talagalay hawl-qabad fudud, oo leh kontorool kombuyuutar oo u gaar ah isticmaalka fudud oo habboon.
Heerkulka Heerkulka: Baadhitaannada heerkulbeegyada laba-geesoodka ah waxay si toos ah u ogaadaan heerkulka kaydinta oo leh cilad khalad ah oo ka yar ± 0.5 ℃.
• Nidaamka Wareegga: Wuxuu la socdaa habka wareegga wareegga wareegga kaas oo si sax ah u koontaroolaya oo kormeeraya heerkulka xalinta, hubinta heerkulka joogtada ah iyo dheelitirka ionic inta lagu jiro electrophoresis.
• Badbaadada Sare: Waxaa ku jira dabool badbaado acrylic ah oo si toos ah u gooya korantada marka la qaado, oo ay la socoto hawlo ilaalin ah oo xad dhaaf ah iyo culays la'aan.
• Heerarka la hagaajin karo: Taangiga elektiroonigga ah iyo tuubada jel waxay ka muuqdaan cagaha la hagaajin karo si loo cabbiro.
• Naqshadaynta Caaryada: Taangiga electrophoresis waxaa lagu sameeyay qaab-dhismeedka caaryada isku dhafan iyada oo aan la isku dhejin;Qalabka korantada waxaa lagu qalabeeyaa 0.5mm electrodes platinum, hubinta adkeysiga iyo natiijooyinka tijaabada deggan.
FAQ
S: Waa maxay pulsed Field Gel Electrophoresis?
J: pulsed Field Gel Electrophoresis waa farsamo loo isticmaalo kala soocida molecules DNA-da waaweyn ee ku salaysan cabbirkooda.Waxay ku lug leedahay beddelka jihada korantada ee matrix gel si ay awood ugu yeelato kala-soocidda jajabyada DNA-da ee aad u weyn in lagu xalliyo agarose gel electrophoresis dhaqameed.
S: Waa maxay codsiyada pulsed Field Gel Electrophoresis?
J: Gel Electrophoresis pulsed Field waxaa si weyn loogu isticmaalaa bayoolaji molecular iyo genetics ee:
Khariidaynta molecules DNA-ga waaweyn, sida koromosoomyada iyo plasmids.
• Go'aaminta cabbirrada genome-ga.
• Barashada kala duwanaanshaha hidde-sideyaasha iyo xiriirka korriinka.
• Epidemiology molecular, gaar ahaan la socodka cudurrada faafa.
• Falanqaynta burburka DNA iyo hagaajinta.
• Go'aaminta jiritaanka hiddo-sideyaal gaar ah ama DNA-da.
S: Sidee buu u shaqeeyaa pulsed Field Gel Electrophoresis?
J: Goobta Gel Electrophoresis ee Pulsed waxay u shaqeysaa iyada oo molecules DNA-ga u gelisa goob koronto oo garaacday oo jihada u beddesha.Tani waxay u oggolaanaysaa unugyo DNA oo waaweyn inay dib isu habeeyaan inta u dhaxaysa garaaca wadnaha, taas oo awood u siinaysa dhaqdhaqaaqooda iyada oo loo marayo matrix gel.Unugyada DNA-da ee yaryar waxay si degdeg ah u dhex maraan jel, halka kuwa waaweyni si tartiib tartiib ah u socdaan, taas oo u oggolaanaysa kala soociddooda ku salaysan cabbirka.
S: Waa maxay mabda'a ka dambeeya pulsed Field Gel Electrophoresis?
J: Goobta Gel Electrophoresis ee Pulsed waxay kala saartaa molecules DNA iyada oo ku saleysan cabbirkooda iyadoo la xakameynayo muddada iyo jihada garaaca wadnaha korontada.Goobta beddelka ah waxay keentaa unugyo DNA oo waaweyn inay si joogto ah dib ugu habeeyaan naftooda, taasoo u horseedaysa u haajirinkooda matrix gelka iyo kala soocida iyadoo loo eegayo cabbirka.
S: Waa maxay faa'iidooyinka pulsed Field Gel Electrophoresis?
J: Xallinta sare ee kala soocida molecules DNA-da waaweyn ilaa dhowr milyan oo lammaane sal ah.Awoodda lagu xallin karo laguna kala saari karo jajabyada DNA-da ee cabbirka isku midka ah
S: Waa maxay qalabka loo baahan yahay ee loo yaqaan "Pulsed Field Gel Electrophoresis"?
J: Goobta la garaaco ee Gel Electrophoresis waxay caadi ahaan u baahan tahay qalab elektrophoresis ah oo leh korantado khaas ah oo soo saara beero garaaca.Agarose gel matrix oo leh feejignaan ku habboon iyo kaydin.Korontada awood u leh in ay dhaliso garaacyada korantada sare.Nidaamka qaboojinta si ay u daadiyaan kulaylka soo baxa inta lagu jiro electrophoresis, iyo bamka wareegga.
