Electrophoresis Wareejinta Dhammaan-in-hal Nidaamka
Tilmaamid
| Tilmaamaha loogu talagalay taangiga Electrophoresis | |
| Cabbirka jel (LxW) | 83×73mm |
| Shanley | 10 ceel (Standard) 15 ceel (ikhtiyaar) |
| Dhumucda shanleyda | 1.0 mm (Standard) 0.75, 1.5 mm (ikhtiyaar) |
| Saxan Kooban | 101×73mm |
| Plate Spacer | 101×82mm |
| Mugga kaydka | 300 ml |
| Tilmaamaha Module wareejinta | |
| Aagga Blotting (LxW) | 100×75mm |
| Tirada Gel-layaasha | 2 |
| Fogaanta Electrode | 4cm |
| Mugga kaydka | 1200ml |
| Tilmaamaha loogu talagalay Bixinta Korontada Electrophoresis | |
| Cabirka (LxWxH) | 315 x 290 x 128mm |
| Korontada soo saarta | 6-600V |
| Soo-saarka Hadda | 4-400mA |
| Awoodda wax soo saarka | 240W |
| Terminalka wax soo saarka | 4 lammaane oo is barbar socda |
Sharaxaada
Nidaamka wareejinta elektrophoresis-ka oo dhan wuxuu ka kooban yahay taangiga electrophoresis oo dabool leh, awood koronto oo leh guddi kontorool, iyo module wareejin ah oo leh koronto. Taangiga electrophoresis waxaa loo isticmaalaa in lagu shubo oo lagu socodsiiyo jel-ka, iyo moduleka wareejinta waxaa loo isticmaalaa in lagu qabto sandwich jel iyo xuubka inta lagu jiro habka wareejinta, waxayna leedahay sanduuqa qaboojinta si looga hortago kulaylka. Korontadu waxay bixisaa korantada hadda loo baahan yahay si ay u socodsiiso jelka oo ay u kaxeyso wareejinta molecules-ka jel ilaa xuubka, waxayna leedahay guddi kontorool oo saaxiibtinimo u leh dejinta electrophoresis iyo xaaladaha wareejinta. Qaabka wareejinta waxaa ku jira electrodes kuwaas oo la geliyo taangiga oo la xiriira jel iyo xuubka, buuxinta wareegga korantada ee loo baahan yahay wareejinta.
Nidaamka wareejinta elektrophoresis-ka oo dhan waa qalab muhiim u ah cilmi-baarayaasha iyo farsamayaqaannada ka shaqeynaya muunado borotiin ah. Nakhshaddeeda is haysta iyo fududaynta adeegsigu waxay ka dhigaysaa mid qiimo leh oo lagu daro shaybaar kasta oo ku lug leh bayoolajiga molecular ama cilmi-baarista biochemistry.
Codsiga
Wareejinta electrophoresis oo dhan-in-hal waa qalab qiimo leh oo ku saabsan berrinka bayoolajiga molecular, gaar ahaan falanqaynta borotiinka. Barootiinada la wareejiyay ayaa markaa lagu ogaadaa iyadoo la isticmaalayo unugyada difaaca jirka ee gaarka ah habka loo yaqaan 'blotting Western'. Farsamadani waxay u oggolaanaysaa cilmi-baarayaasha inay aqoonsadaan borotiinno gaar ah oo xiisaha leh oo ay qiyaasaan heerarkooda muujinta.
Sifaysan
• Alaabtaku habboon cabbir yar PAGE jel electrophoresis;
• Alaabta's xuduudaha, qalabyada ayaa si buuxda ula jaan qaadaya alaabada caanka ah ee suuqa;
•Qaab-dhismeedka sare iyo naqshad jilicsan;
• Hubi saamaynta tijaabada ku habboon laga bilaabo shubista jel ilaa orodka jel;
•Si deg deg ah u wareeji jelyada cabbirka yar;
•Laba cajaladood oo jeel ah ayaa lagu ridi karaa haanta;
•Waxay socon kartaa ilaa 2 jel hal saac gudaheed. Waxay ka shaqayn kartaa habeen ka dib wareejinta xoojinta hoose;
• Cajalado jal-haysta oo midabyo kala duwan leh waxay xaqiijinayaan meelaynta saxda ah.
FAQ
S: Waa maxay wareejinta elektrophoresis-ku-hal-hal ee loo isticmaalo?
J: Isku-gudbinta elektrophoresis-ka oo dhan-in-hal waxaa loo adeegsadaa ka wareejinta borotiinnada jel-ka polyacrylamide ee xuubka si loo sii baaro, sida xoqidda Galbeedka.
S: Waa maxay xajmiga jel ee la samayn karo oo lagu wareejin karo iyada oo la adeegsanayo wareejinta electrophoresis dhammaan-in-hal?
A: nidaamka wareejinta elektrophoresis-ka oo dhan wuxuu ku shubi karaa oo ordi karaa cabbirka jel 83X73cm ee tuurista gacanta, iyo 86X68cm jel-kabka hore. Aagga wareejintu waa 100X75cm.
S: Sidee buu u shaqeeyaa habka wareejinta elektrophoresis-ka?
J: Isku-gudbinta elektrophoresis-ku-dhammaan-ku-jirka ah waxay isticmaashaa electrophoresis si ay borotiinada uga wareejiso jel ilaa xuubka. Barootiinada marka hore waxaa lagu kala soocaa cabbir ahaan iyadoo la isticmaalayo polyacrylamide gel electrophoresis (PAGE) ka dibna loo wareejiyaa xuubka iyadoo la adeegsanayo goob koronto.
S: Waa maxay nooca xuubka loo isticmaali karaa nidaamka wareejinta elektrophoresis?
J: Noocyada kala duwan ee xuubka ayaa loo isticmaali karaa wareejinta elektrophoresis ee dhammaan-in-hal nidaamka oo ay ku jiraan nitrocellulose iyo PVDF (polyvinylidene difluoride).
S: Ma loo isticmaali karaa dhammaan habka-gudbinta elektrophoresis-ka si loo baaro DNA-da?
J: Maya, Nidaamka wareejinta elektrophoresis-ka oo dhan waxaa loogu talagalay si gaar ah falanqaynta borotiinka loomana isticmaali karo falanqaynta DNA-da.
S: Waa maxay faa'iidooyinka isticmaalka electrophoresis wareejinta dhammaan-in-hal nidaamka?
A: Nidaamka wareejinta elektrophoresis-ka oo dhan wuxuu u oggolaanayaa wareejinta hufan ee borotiinnada laga bilaabo jel ilaa xuub, siinta dareen sare iyo gaar ahaan ogaanshaha borotiinka. Sidoo kale waa hab ku haboon oo dhan-in-hal kaas oo fududeynaya habka xoqidda reer galbeedka.
S: Sidee loo ilaalin karaa dhammaan nidaamka wareejinta elektrophoresis?
J: Xawilaadda elektrophoresis-ka oo dhan waa la nadiifiyaa isticmaal kasta ka dib waxaana lagu kaydiyaa meel nadiif ah oo qalalan. Electrodes-ka iyo qaybaha kale waa in si joogto ah loo hubiyo wixii waxyeelo ah ama daal iyo dillaac ah.
