DNA jel electrophoresis waa farsamo bayooloji molecular caadi ah oo loo isticmaalo kala soocida iyo falanqaynta jajabyada DNA-da oo ku salaysan cabbirkooda.Nidaamku wuxuu ku lug leeyahay in lagu raro jajabyo DNA oo kala duwan oo lagu dhejiyo jel ka samaysan agarose, karbohaydrayt laga helo algae cas.
Diyaarinta iyo tuurista jelka agarose
- Ku milaan agarose qadar habboon oo ah kaydka elektrophoresis.Isku-duubnaanta jel waxaa lagu go'aamiyaa saamiga tirada-ilaa-mugga, sida 1g of agarose ee 100.ml kaydka 1% jel.
- Kuleyli isku dhafka microwave-ka, ku wareejin weelka si aad u hubiso inuu si buuxda u milmo agarose.
- Ku dar ethidium bromide xalka jel ee ugu dambeeya ee 0.5mg/ ml.Ethidium bromide wuxuu u dhexeeyaa lammaane DNA ah oo ku xiga wuxuuna sii daayaa fluorescence orange hoos iftiinka UV.Ogsoonow in ethidium bromide uu yahay kansar, markaa la tacaalida waxay u baahan tahay tallaabooyin badbaado oo habboon sida xidhashada galoofyada.
- Ku qabooji xalalka jel ee qubeyska biyaha si aad uga hortagto saxaarada jelku in ay soo noqoto heerkulka sare awgeed.
- Shanlada geli xalka jel si aad u samaysato ceelal muunad ah.Dooro shanlooyinka ku habboon qaddarka saamiga DNA-ga ee aad ku shubayso.Ku shub xalalka agarose ee gudahasaxaarad jeluna ogolow inay ku xoojiso heerkulka qolka.
- Marka jelku adkeeyo, ka saar shanlada.Haddii aadan isla markiiba isticmaali doonin jelka, ku duub duub balaastig ah oo ku kaydi 4 ℃ ilaa loo baahdo.
Diyaarinta iyo socodsiinta jeelka
Kahor intaadan bilaabin electrophoresis, ku qas muunada DNA-ga iyo wax kaydinta.Bakhaar-ku-saarku wuxuu badiyaa lix jeer ka badan yahay xoogga wuxuuna ka caawiyaa muunada inay hoos u degto ceelasha hoose oo ay la socoto dhaqdhaqaaqa inta lagu jiro electrophoresis.
U deji sahayda tamarta korantada la cayimay.
Ku dar bakhaar ku filan elektrophoresis taangiga jel si uu u dado dusha jelka.Hubiisku xirka korantada saxda ah.
Ku shub muunadda DNA-da iyo calamadaha miisaanka unugga ee ceelasha jelka.
Daar korontada si aad u bilowdo electrophoresis.
U fiirsashada jajabyada DNA ee kala soocan
Ka dib electrophoresis, dami korontada oo ka saar jel.
Isticmaal isha iftiinka UV si aad u iftiimiso jelka;Qaybaha DNA-da waxay u muuqan doonaan sida xargaha fluorescent orange.
Qor sawirka jel si aad u falanqeyso jajabyada DNA-da ee kala go'ay.
Tijaabada ka dib, hubi in si sax ah loo qubo jel iyo qalab elektrophoresis ah, iyadoo la raacayo tilmaamaha shaybaarka.Had iyo jeer xidho galoofyada marka aad la tacaalayso jel-ka iyo bakhaarada ay ku jiraan ethidium bromide si aad uga ilaaliso soo-gaadhista walaxdan khatarta ah.
Electrophoresis-ka DNA-ga waxaa si weyn loogu isticmaalaa bayoolajiga molecular iyo cilmi-baarista hidde-sidaha si loo qiyaaso cabbirrada molecule DNA-ga, kala-soocidda jajabyada DNA-da, ogaanshaha isbeddellada hidda-wadaha, iyo sawir-qaadista DNA-da, iyo codsiyada kale.Waa farsamo tijaabo ah oo fudud oo wax ku ool ah oo ka caawinaysa fahamka ka kooban iyo sifooyinka muunadaha DNA-da.
Beijing Liuyi Biotechnology Co.Ltd, soo saare xirfad leh oo diiradda saaraya alaabada electrophoresis ka badan 50 sano, waxay bixisaa noocyo kala duwan oo taangiyada electrophoresis (qolalka / unugyada) ee jel electrophoresis, sida taangiyada electrophoresis siman (qolalka / unugyada)DNAjel electrophoresis, iyo taangiyada electrophoresis taagan (qolal/unugyada) ee borotiinka jel electrophoresis.Dhanka kale, waxay sidoo kale bixisaa noocyo kala duwan oo koronto koronto ah, golaha golaha mugdiga ah ee sanduuqa mugdiga ah iyo transilluminator UV.Waxaad dooran kartaa taangiyada elektrophoresis ee Beijing Liuyi (qolalka/unugyada) si aad u cabbirto miisaanka kelli ee RNA oo aad u kala saarto RNA oo cabbirro kala duwan leh shaybaarkaaga.
Halkan waxaan sameyn doonaaku talinnooc ka mid ah taangiga elektrophoresis jiifka ah (qolka / unugga) modelDYCP-31DN si ay u sameeyaan oo u ordaan gel.
Kadib socodsiinta jel-ka, waxaad isticmaali kartaa sawirka gelitaanka iyo qaabka nidaamka falanqayntaWD-9413Bsi ay u fiirsadaan, u falanqeeyaan oo ay sawiro u qaataan jel.Liuyi Biotech waxay kaloo bixisaa UV transilluminator (UV Analyzer) si loo ilaaliyo jelka.Waxaan haynaa nooca sanduuqa madow ee UV transilluminator (UV Analyzer).WD-9403A,9403C,WD-9403F, nooca UV transilluminator (UV Analyzer) ee la qaadan karoWD-9403Biyo gacan ku haynta UV transilluminator (UV Analyzer)WD-9403Eadiga dooro.
Astaanta Beijing Liuyi waxay leedahay in ka badan 50-sano oo taariikh ah Shiinaha shirkadduna waxay ku siin kartaa alaab deggan oo tayo sare leh adduunka oo dhan.Inta lagu jiro sannadaha horumarinta, waxay u qalantaa doorashadaada!
Waxaan hadda raadineynaa la-hawlgalayaal, labadaba OEM electrophoresis taangiga iyo qaybiyeyaasha waa la soo dhaweynayaa.
Haddii aad leedahay wax qorshe ah oo wax iibsiga ah oo alaabtayada ah, fadlan ha ka waaban inaad nala soo xidhiidho.Waxaad fariin noogu soo diri kartaa iimaylka[email protected]ama[email protected], ama fadlan naga soo wac +86 15810650221 ama ku dar Whatsapp +86 15810650221, ama Wechat: 15810650221.
Waqtiga boostada: Agoosto-08-2023