Mabda'a
Cellulose acetate film electrophoresis waa habka electrophoresis iyadoo la isticmaalayo filim cellulose acetate taageero ahaan.Dhacdada ay walxaha la dallacay u dhaqaaqaan dhanka korantada ka soo horjeeda ee hoos timaada falgalka beerta korontada waxaa loo yaqaan electrophoresis.Maadaama borotiin kasta uu leeyahay bar isoelectric gaar ah, haddii borotiinka lagu dhejiyo xal leh pH ka hooseeya barta isoelectric, borotiinku si togan ayaa loo dallaci doonaa oo u dhaqaaqi doonaa korantada taban.Taa lidkeeda, waxay u guurtaa tiirka togan.Sababtoo ah xawaaraha molecules-ka borotiinka ee ku socda beerta korantada waxay la xiriirtaa kharashkooda, qaabka iyo xajmiga molecules, borotiinnada kala duwan waxaa lagu kala saari karaa electrophoresis.Serum wuxuu ka kooban yahay borotiinno kala duwan, kuwaas oo dhammaantood leh dhibco isoelectric ee pH7.5 ama ka yar.Marka serum la dhigo pH 8.6 baffer si loo ordo electrophoresis, dhammaan borotiinada serum si xun ayaa loo dallacay waxayna u dhaqaaqi doonaan dhinaca togan ee beerta korantada.Maadaama borotiinada serum kala duwan ay leeyihiin kharashyo kala duwan isla pH isku mid ah, qaybaha molecular way kala duwan yihiin cabbirkooda, sidaas awgeed xawaaraha socdaalka wuu ka duwan yahay, waxaana kala soocaya electrophoresis.Dhibcaha isoelectric iyo miisaanka molecular ee borotiinka serum ayaa lagu muujiyay shaxda hoose:
| Protein | Dhibcaha korontadu (PI) | Miisaanka molecular |
| Albumin | 4.88 | 69 000 |
| α1-gloulin | 5.00 | 200 000 |
| α2-gloulin | 5.06 | 300 000 |
| β-gloulin | 5.12 | 9 000 ~ 150 000 |
| γ-gloulin | 6.85 ~ 7.50 | 156 000 ~ 300 000 |
Tijaabadu waa in la kala saaro borotiinno kala duwan oo ku jira serumka dhiigga oo leh xuubka acetate cellulose (abbr. CAM) oo ah warbaahinta taageerada.CAM waa nooc ka mid ah loos xumbo lehefilim leh lebis wanaagsan, dhumucdiisuna waa 0.1mm-1.5mm, kaas oo leh nuugista biyaha qaarkood.
Qalabka, qalabka iyo reagents ee CAM Electrophoresis
Tusaalooyinka:serum dhiiga dadka oo caafimaad qaba
Qalabka:korontada DYY-6C, taangiga electrophoresis DYCP-38C, muunad sare oo loading WD-9404
Reagents
1) xuubka acetate cellulose 7X9cm
2) PH 8.6 barbitol baffer xal (awood ionic 0.05-0.09, waa 0.06tid time.): qaado 1,84g Diethyl barbituric acid, ka dibna qaado 1.03g Diethyl sodium pentobarbital, ku dar qaar ka mid ah biyo diiray si aad u kululaato si ay u milaan, oo u samee. ilaa 1000 ml;
3) Wasaaq: Ponceau S 0.2g, Trichloroacetic 3g, ku dar 100ml biyo saafi ah;
4) TBS / T ama PBS / T: 45ml 95% ethanol, 5ml glacial acetic acid, ku dar 50ml biyo nadiif ah;
5) Xalka Nadiifinta: 70ml ethanol anhydrous, 30ml glacial acetic acid.
Habka tijaabadad
1) Diyaari xuubka: Geli xuubka 30min-8h ee barbitol baffer, oo ka soo saar, ka saar xalka dheeraadka ah adigoo isticmaalaya warqad nuugista.
2) Tijaabooyin soo dejineed: Kala saar dhinaca qallafsan iyo dhinaca siman ee xuubka, oo calaamadee xariiq masaafo 1.5cm ah ilaa cidhifka sare ee dhinaca qallafsan.Ku shub muunadaha iyadoo la isticmaalayo muunad saraysa oo lagu raraynayo dhinaca xun.Fiiro gaar ah: Tijaabooyinku waa in lagu raro dhinaca xuubka ee qallafsan.Ka dib markii shaybaarka serum-ka si buuxda loo geliyo xuubka, ka rog xuubka, dhig dhinaca qallafsan (samballada) hoos u dhig taangiga, dhammaadka muunada waxaa lagu dhejiyaa korantada taban.
3) Electrophoresis: Daar korontada, 0.4～0.6m A/cm xuub, wakhtiga orodku waa 30-45 min.Kadib markaad ordo electrophoresis, dami korontada.
4) Wasakh oo nadiif ah: Ka saar xuubka taangiga, oo quusiit geli xalka dheeha 5 daqiiqo, ka dibna ku nadiifi xalka nadiifinta marar badan 3-4 jeer ilaa midabka asalka ahi cad yahay.Borotiinnada serumka waa in lagu muujiyaa xargaha, caadi ahaanna waxaa jira shan aag, oo ka samaysan qaybta ugu sarreysa ee xariiqda calaamadeysan, Albumin, α1-gloulin, α2-gloulin, β-gloulin, γ-gloulin.
5) Ilaalinta: dhig elecrtophoresis qalalanbandxalinta nadiifinta 10-15 daqiiqo, ka dibna ka saar oo ku dheji galaas nadiif ah, ka dib marka ay qallajiso, waxay noqon doontaa filim hufan.band.
TijaabiNatiijo
Saamaynta kala-soocidda ee shaybaarrada serumku waa wanaagsan tahay, mana jirto wax ifafaale ah oo dabada ah.Dib-u-celinta natiijadu way kala duwan tahay iyadoo ay ugu wacan tahay hababka tijaabada iyo hababka tijaabiyaha, iyo dib u soo celinta ayaa sarreeya.
Gabagabo
Nidaamka ogaanshaha elektrophoresis ee degdegga ah ee bukaan-socodkaTaangiga ElectrophoresisDYCP-38C,korontadaDYY-6C iyo muunado sarsare oo rarida WD-9404) oo ay soo saartay ourShirkadda Beijing Liuyi Biotechnology Co., Ltdbuuxiyey shuruudaha tijaabada,iyoNatiijooyinka waa la soo saari karaa, fudud oo degdeg ah, ku habboonwaxbariddacilmi baaris.
Beijing LiuyiBiotechnology Co., Ltd waxay ku takhasustay soo saarista alaabada elecrtophoresis ee warshadaha sayniska nolosha, kuwaas oowaxay leedahay in ka badan 50-sano oo taariikh ah Shiinaha shirkadduna waxay ku siin kartaa alaab deggan oo tayo sare leh adduunka oo dhan.Inta lagu jiro sannadaha horumarinta, waxay u qalantaa doorashadaada!
Waxaan hadda raadineynaa la-hawlgalayaal, labadaba OEM electrophoresis taangiga iyo qaybiyeyaasha waa la soo dhaweynayaa.
Wixii macluumaad dheeraad ah oo nagu saabsan, fadlan nagala soo xiriir email ahaan[email protected]ama[email protected].
Waqtiga boostada: Nov-14-2022