Sida loo sameeyo DNA Electrophoresis ee Agarose Gel?

Halkan waxaan ku tilmaami doonaa sida loo sameeyo agarose gel electrophoresis by cilmi-baadhahayaga shaybaarka Liuyi Biotech's.

Tijaabada ka hor, waxaan u baahanahay inaan hubino qalabka, reagents, iyo agabka kale ee tijaabada ah ee aan u baahanahay.

Diyaarinta qalabka tijaabada ah iyo agabka

Qalabka loo yaqaan 'agarose gel electrophoresis'

Horizontal electrophoresis unug (taangi / qol), dhexe iyo hoose korantada korantada electrophoresis, image jel & nidaamka falanqaynta.

Beijing liuyi Biotechnology Co., Ltd (Liuyi Biotech) waxay bixisaa noocyo kala duwan oo ah unug elektrophoresis jiifa (taangi / qol) iyo koronto iyo sidoo kale nidaamka dukumentiyada jel.Moodeelka DYCP-31 alaabooyinka taxanaha ah waxaa loogu talagalay electrophoresis, iyo alaabada taxanaha DYY waa sahayda korantada.Alaabooyinka taxanaha WD-9413 waa nidaamka dukumeentiyada jel.Laga soo bilaabo cabbirka gel yar ee 60 × 60mm ilaa jel weyn 250 × 250mm, waxaan buuxin karnaa shuruudahaaga kala duwan ee cabbirka jelka.QaabkaDYCP-32Cwuxuu ka dhigi karaa jel si uu u gaaro cabbirka 250×250mm.TheDHAQAN-6Cwaa korontodeena.Waxay taageertaa wax soo saarka 400V, 400mA, 240W, kaas oo ah badeecadayada caadiga ah ee ay isticmaalaan macaamiisheena. WD-9413BSawirka Gel & Nidaamka Falanqaynta waxaa loo isticmaalaa falanqaynta iyo cilmi-baarista jel, filimaanta, iyo boogaha ka dib tijaabada elektrophoresis.Waa aalad aasaasi ah oo leh isha iftiinka ultraviolet si loogu sawiro loona sawiro jeelyada lagu dheehdo dheeha fluorescent sida ethidium bromide, iyo il cad oo iftiin leh oo lagu sawiro oo lagu sawiro jeelyada leh midabyo sida coomassie dhalaalaya buluug.

Reagents-ka loo yaqaan 'agarose gel electrophoresis'

1.Dadka dhexe: agaroose jel

2.Qofka: TAE (Tris-acetate, EDTA, glacial acetic acid) iyo TBE (Tris-borate, EDTA, boric acid).

3. Bakhaarka xajinta: 6×DNA Loading Buffer (Gaar u ah muunadda DNA: EDAT, glycerine, Xylene cyanol iyo bromophenol blue)

Midabka

Midabka fluorescent sida EB, Gelred, goldview, GenGreen, GenView, SYBRGreen

TheAlaabta la isticmaalo

Talooyinka pipette-ka jeermiska jeermiska (10μL), talooyinka pipette (200μL), talooyinka pipette (1000μL), 200μL \ 500μL \ 1.5ml tuubo sentifuge.

Calaamadaha DNA

Waxaa loo diyaariyey iyadoo loo eegayo miisaanka molecular in la tijaabiyo.

Gelku ordayaa oo u fiirsada

Marka hore, waxaan u baahanahay inaan diyaarino jel agarose.Isku-duubnaanta agarose-ka ee jel-ka waxay ku xirnaan doontaa cabbirrada jajabyada DNA-da ee la kala saarayo, iyada oo inta badan gelyada ay u dhexeeyaan 0.5%-2%.QaadashadayadaDYCP-31DNtusaale ahaan, Waxaad tuuri kartaa jel yar yar, jel ballaaran , jel dheer iyo jel square sida waafaqsan shuruudaha tijaabada.Dooro saxaarad jel ah oo aad u baahan tahay, oo geli shanlada, ka dibna ku shub jelka agarose-ka ee kulaylka leh aaladda jelka wax lagu shubo.

Dabadeed, ka dib markii jelku adkaado, si taxadar leh oo jilicsan uga qaad shanlada, ka dibna geli saxamada jel haanta kaydinta.Ku shub xalka kaydka taangiga kaydka ah oo ka dhig dhammaan jel la dhex geliyo kaydka.Ku shub muunada ceelasha oo leh tuubo caadi ah.Isku xidhkaDYCP-31DNoo leh koronto-korontada elektiroonigga ah si sax ah, oo u deji xuduudaha si ay u socodsiiso jelka.

U sawir jajabyada DNA-gaaga oo ku jira transilluminator UV.

AKadib socodsiinta jel-ka, waxaad isticmaali kartaa sawirka gelitaanka iyo qaabka nidaamka falanqayntaWD-9413Bsi ay u fiirsadaan, u falanqeeyaan oo ay sawiro u qaataan jel.Liuyi Biotech waxay kaloo bixisaa UV transilluminator (UV Analyzer) si loo ilaaliyo jelka.Waxaan haynaa nooca sanduuqa madow ee UV trmoodelka ansilluminator (UV Analyzer).WD-9403A, 9403C, WD-9403F, nooca UV transilluminator (UV Analyzer) ee la qaadan karoWD-9403Biyo gacan ku haynta UV transilluminator (UV Analyzer)WD-9403Eadiga dooro.

Sawirka jel ka dambeeya electrophoresis

Astaanta Liuyi waxay leedahay in ka badan 50 sano oo taariikh ah Shiinaha shirkadduna waxay ku siin kartaa alaab deggan oo tayo sare leh adduunka oo dhan.Inta lagu jiro sannadaha horumarinta, waxay u qalantaa doorashadaada!

Wixii macluumaad dheeraad ah oo nagu saabsan, fadlan nagala soo xiriir email ahaan[email protected], [email protected].